• No Comments

ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

Author: Gonos Dairr
Country: Ghana
Language: English (Spanish)
Genre: Medical
Published (Last): 15 July 2012
Pages: 402
PDF File Size: 18.97 Mb
ePub File Size: 18.15 Mb
ISBN: 875-2-19766-595-8
Downloads: 16355
Price: Free* [*Free Regsitration Required]
Uploader: Mazulkis

Proteins in the latex migrate to the surface and penetrate through the skin. Glove manufacturers try to avoid powder by producing a powder-free or powder-reduced glove. Many efforts have been made to remove the proteins. Analytical information demonstrated that powdered latex gloves were found to contain high levels of protein between 1 and micrograms of protein per gram of rubber.

Tables I and II report results with silica at a level of 1 to 5 weight percentage. It is well known that proteins can transfer from the latex to the powder. The proteins probably would stay adsorbed on the latex particles throughout the compounding process. The proteins then easily are removed. It is suggested that the silica attaches itself to the surface of the latex particle substituting the protein molecules and displacing them, see Fig.

Navigation Top Stories Search. Further analysis by the LEAP method also at Guthrie Research, resulted in clean films having protein values below the detection level, 0. Such powder-free films might have a different kind of a problem. A general estimate is that only about 20 percent of the total protein evaluated by the ASTM test are harmful.

D Specification for Rubber Examination Gloves. As a consequence of the addition of ammonia for preserving the latex concentrate, the lipid materials are believed to hydrolyze slowly, releasing fatty acids.

Allergy and Clinical Immunology, 58, FORMAT Single User Subscription Site License Subscription BOOKS All Books PACKAGES All Packages EDITION to PUBLISHER.



A previous paper on cleaning latex films with fumed silica5 presented protein results derived by ASTM D Summary and conclusions Cost and process advantage of latex dipped with fumed silica Presently, latex goods with relatively low protein content are being manufactured by various complicated methods. This problem has become more apparent in the last several years due to the increased use of rubber gloves for infectious diseases protection. However, traces still may remain and cause skin reactions.

Thompson et al9 that there is a relationship between hydration and compromised barriers in some surgical gloves. However, the low silica concentrations necessary make this a cost-effective process for addressing protein allergy concerns. If you like to setup a quick demo, let us know at support madcad.

Latex Protein Analysis

The silica dipping process is simple, short and inexpensive. The test method involves an extraction and precipitation procedure followed by an assay of protein content.

Here again, the presence of silica greatly diminished the level of latex allergenic proteins measured by the LEAP method. A non-silica rubber blank has micrograms of proteins per gram of rubber, see Table I.

This paper suggests a way to eliminate the proteins with a simple, elegant and inexpensive method using fumed silica additives. The following editions for this book are also available No items in cart. This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Another method of limiting protein migration is by coating the natural latex surface with a polymer barrier which also provides smoothness.

Generally I have found correlation between the two tests. There were no books found for the applied search filters.

Introduction The problem of proteins in natural rubber Type I latex allergies can be caused by proteins in the natural latex used in gloves, condoms, balloons and other articles.


Testing procedures required, please see the full standard for complete details. Enter your personal account email address to request a password reset: Experimental results and discussion Low-protein allergen analysis in latex compounded with fumed silica Compounding of fumed silica dispersion for dipped natural latex application resulted in tensile and tear enhancement as described before. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.

The extracted protein is atm and quantified colorimetrically by the modified Lowry method using a protein standard.

ASTM D5712 – 15

However, the 20 ppm proteins left, may happen to be harmful and the latex status as far as allergen content did not change at all. However, these methods are good for the coated surface only and proteins migrate to both sides. Table III shows results with silica at a lower level of 0. Historically latex gloves are powdered for two reasons. Two-sided coating is possible but expensive.

Please login to your authorized staff account to use this feature. This problem has been publicized in the media and described in the literature. It was found that the addition of fumed silica to latex compounds helps in manufacturing of cleaner latex goods with lower protein extractables.

Natural Rubber Science and Technology, A. Powder-free latex gloves and other methods of reducing protein content Latex gloves are among the most commonly used critical latex articles. That’ll take 30 years For autonomous satm to gain passengers’ trust, communication is key ‘Impatient’ Mary Barra reawakens spirit of crisis at GM Nitto invests to understand, better serve its customers Self-driving cars might make people sick to their stomachs.